Chapter 156: Microscope
All are given, all are given, don’t sprinkle milk, this thing is expensive.”
As Achang shouted, several people carried a bucket of milk from the room, and a piece of glass that was buttoned on it was taken off. Achang touched the wall of the bucket, and the temperature was lower than the temperature on his hands.
"Xiao Liuzi, go call Master, and say that the rice paste and milk are ready, and the temperature is right."
Xiao Liuzi just ran to the door, and before he could go upstairs, Zhou Heng brought his people down, he walked quickly towards Achang's position.
Zhou Heng looked at it and found that the rice paste was very sticky. There was a layer of milk skin floating on the milk, which looked very thick. There were a lot of both kinds of things, including two large buckets. Seeing a box of Petri dishes behind, Zhou Heng waved his hand.
The conditions are limited, Zhou Heng wanted to make a culture medium that is more suitable for Penicillium growth. After all, there is no Chaser agar, no Chaser yeast paste agar, no wort agar, and no beer wort agar.
So we can only make the original culture medium, but the effect is still no different.
"Move all these things to a small room behind the first floor. I will teach you how to make culture medium. In the future, I will make it according to this method. Now that the weather is cold, it can be stored for more than ten days without any corruption."
Achang quickly brought people to carry these things into the room, and of course, there were no plates of penicillium plaques left.
"Go and find me a few pieces of gauze that are three feet by three feet square, and you need both roving and yarn, and then bring me a bucket of distilled water and a few basins."
Qu Zi was quick-witted and had already brought people to get it, and he took it back soon.
Zhou Heng poured a plate of Penicillium into a basin, and then someone poured in distilled water. In an instant, the green mold in the basin floated up.
Hand a long spoon to Xiao Liuzi, "Don't be impatient, stir slowly, let these molds dissolve in the water and then filter, add water and stir again, then filter again, do you understand?"
Xiao Liuzi nodded, took two people and made three basins, and began to stir slowly. His movements were extremely gentle. After all, they were all made by picking up medicine, with quick hands and feet and accurate heads. People around him watched their movements without blinking.
Zhou Heng looked at A Chang with a smile and walked towards him, put a toxic kettle on the table. Zhou Heng measured the rice paste, taro juice and milk one to one with a large beaker and poured it into the pot.
Then he held the kettle, stirred it evenly, picked up a Petri dish, poured the natural nutrient solution into it, and it seemed that the temperature was getting lower and lower, and the juice had become extremely viscous. After pouring it in for a while, it was semi-coagulated like oil and attached to the bottom of the Petri dish.
Zhou Heng handed the kettle to Achang, "Just pour one third of the Petri dish one by one. The principle is to lay a layer flat, and then don't shake and stop a cup of tea. It will solidify. Just make fifty ones, and then find a place with the lowest temperature to store."
Achang took the kettle and his hands did not stop, but his face still seemed a little confused.
"Master, can these rice soups be used as medicine?"
Zhou Heng shook his head, "It's useless to say it now. You will know it later. Hurry up and do something else."
Achang quickly started making the Petri dish without stopping. Zhou Heng got close to Xiao Liuzi and the others. The mold had quickly dissolved into the water. The color inside the water turned green, but there were still many large pieces of impurities.
"Okay, stop stirring."
Xiao Liuzi stopped and Zhou Heng glanced at the liquid and glanced at the gauze.
"Find the thickest gauze, filter it for the first time, and filter out the flesh and large pieces of impurities."
Xiao Liuzi nodded, picked up the basin, poured the liquid into the basin covered with a layer of gauze. Large pieces of flesh were left on the gauze, but the penicillium plaque was rarely left.
Soon several large basins underwent preliminary rough filtration like this. Zhou Heng looked at the liquid. The concentration of the turbid blue liquid was still very high, and a smile appeared on his face.
He raised his eyes and continued, "Find some such small water tanks for me, the kind that has been disinfected before."
Xiao Liuzi quickly brought people to bring some stone pottery jars for pharmaceutical products. Zhou Heng pointed to the basin and ordered:
"Populate these mixed liquid into the tank, remember to pour one third of it, then pour one third of the nutrient solution made by Achang, cover the tank with a white cloth, tie it tightly with a rope, and send someone to the next door of the alcohol room, where the temperature is high and it is easy to ferment and suitable for mold growth. It takes seven days for the low temperature environment. If it is next door to the alcohol room, it can be purified in four or five days."
Without giving instructions, a few servants stepped forward and carried more than a dozen large tanks toward the next door of the alcohol room. Achang did not forget to shout from behind:
"Go to find Brother Xue, we bought the lock and lock the room later."
At this moment, the window on the second floor poked out, and Ma Lingshan called Zhou Heng excitedly.
"Master, the microscope seems to be available this time."
Zhou Heng was happy on his face, and quickly walked upstairs and returned to the room where he was doing research. Liu Xiu'er quickly made way for him.
Ma Lingshan had already introduced anxiously, "Junior sister and I debugged it again just now and found that the lenses we installed were in the opposite direction, so we took them apart and reinstalled them. This time, Master, please take a look, can you see clearly?"
Zhou Heng got closer and looked at it with a low-power lens. There was a mold the size of a needle tip on the two slides. Looking at it from the low-power lens, Zhou Heng soon found the position. Not to mention the good magnitude, Zhou Heng adjusted to the position of the high-power lens. This was a small dot where the mold could be seen, and Zhou Heng was a little excited.
Unexpectedly, based on their own memory, they actually made such a microscope diagram with a lot of loopholes. Judging from the plural, this high-power mirror should reach more than 400 times. As for how many of them are, it is hard to say.
Zhou Heng found a small bottle with grease inside.
Even if the microscope they made was a high-power mirror, the magnification was still limited, so they could only use the oil mirror to increase the magnification. However, these were all the information that Zhou Heng checked when he was in school, and he didn't know if it could be realized.
Taking a deep breath, lowered the oil on the outer layer of the slide. Zhou Heng adjusted the position of the oil mirror and slowly moved down. In an instant, there were still small molds in the eyepiece, which were enlarged. One by one, as if the branches-shaped mycelium appeared in front of him, Zhou Heng was a little excited and let the position of the microscope go.
"It's successful, you can come and have a look."
Ma Lingshan hurriedly came up, saw the mycelium, with a look of confusion on his face, then looked at the slide, widened his eyes and looked in disbelief.
"Master, this is the small dot on the glass sheet, which can actually be enlarged into a branch. How many times has it been enlarged?"
Zhou Heng picked up the pen and made a simple calculation on the booklet.
"If I had a good calculation, this should have been expanded by 1,600 times."
Mingyu, behind Boss Xue, leaned over and saw that Desheng and Liu Xiu'er had finished watching, so he quickly stepped forward.
Put your eyes on the eyepiece, look at the mycelium below, staring at it without blinking, and you don’t know what you are thinking in your mind.
Suddenly Mingyu exclaimed, "Oh, why does this thing move? I just saw a small bubble appearing in the corner of the branch. Is it growing?"
Mingyu's words made Zhou Heng stunned. Unexpectedly, Mingyu was the first to discover that this thing was alive.
It seems that reading more is useful, but educated people are different.
"By the way, this thing is called Penicillium, it is alive. If you are interested, you can find a patient who has been infected for many days of trauma, take some secretions from his wounds, and then apply two boxes on the Petri dish. One box is not treated, and the other box is applied with a layer of Penicillium such as Penicillium on it. After a few hours, you can observe and see what changes are happening to these molds."
This time, Ma Lingshan raised his hand and said, "I'll go find the patient. An old man came yesterday. His son's leg was broken. He had found many clinics before and could not be treated. He said that his calf had swollen and pus and had flies. I asked him to come here early this morning for medical treatment. I don't know if he came."
As soon as he finished speaking, Qu Ziping hurriedly pushed the door open and came in.
"Boss, a patient came outside Dr. Ma. If you have a leg injury and someone is carrying him down and take a look."
Zhou Heng paused and looked at Ma Lingshan, who quickly stood up.
"It seems that this person is the son of the old man yesterday. Master, I'll go down and take a look first."
Zhou Heng called Ma Lingshan, "Wait, let's go together, Xiu'er will follow you too."
Liu Xiu'er was stunned, her eyes instantly panicked.
"Ah, I'll go too?"
Chapter completed!